BioDefense
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Mutation Discovery
  - LightScanner
  - LightScanner 32
  - LightScanner Master Mixes
  - LCGreen Plus Melting Dye
  - LightScanner Assays
  - Custom Probes & Primers
  - Reagents, Buffers & Capillary Tubes
Genotyping
FilmArray
LightScanner® 32 System

The LightScanner 32 (LS32™) instrument is an air thermocycler capable of automatically analyzing samples for the presence of targeted nucleic acid. It combines real-time, rapid thermocycling technology and Hi-Res Melting and is capable of analyzing 32 samples per run in less than 60 min. The LS32 instrument performs for three-color detection for real-time and single-color detection for Hi-Res Melting. Real-Time PCR results can be generated using a variety of labeled probe chemistry including SimpleProbe®, FRET probes or HybProbe®. Optimal Hi-Res Melting results are generated using LCGreen® Plus dye.

Like the LightScanner, the LS32 is unsurpassed in its temperature control and data acquisition rates. With superb sensitivity and specificity, this instrument is designed to meet the needs of high throughput scanning projects. Intuitive analysis software groups the results and provides automatic results.

Benefits from a Complete Product Solution

Instrument   Chemistry
  Results in thirty minutes   Optimized Master Mix for superior PCR performance for Hi-Res Melting applications
  Hi-Res Melting is non-destructive allowing samples to be recovered for further analysis    
Support Software
  Obtain quick assistance through phone and online help desks   Software automatically analyses each sample and defines the standards and variants
  Work directly with highly experienced scientists   Simple data management tools
  Primer design software
 

LightScanner 32 Software

LS32 SoftwareA computer is included with the system that comes pre-loaded with easy-to-use run and analysis software. The software automatically collects and interprets data, then reports the results. This software also provides users with simple data management tools.

 

 

 

 

The Fluorimeter Component

A three-channel fluorimeter block is used for the real-time detection of the dyes/probes in each amplification reaction. The fluorimeter reads and stores data for each sample simultaneously in all three channels with every fluorescence acquisition. Excitation of each sample is from a light emitting diode (LED) that is filter tuned and passed through a dichroic mirror at 470 nm. The three detection channels are filter tuned and correspond to 530 nm, 640 nm, and 710 nm, respectively.

The Fluorimeter Component

LightScanner 32 and Computer Specifications

Sample Description
Sample capacity: 10 µL samples
32 sample carousel

Fluorescence Acquisition for Real Time PCR
Blue LED excitation with peak emission of 470 nm
3 channel detection at 530 nm, 640 nm, and 705 nm

Fluorescence Acquisition for Hi-Res Melting (Highly optimized for LCGreen Plus)
Blue LED excitation with peak emission of 450 nm
Single channel detection at 500 nm

Performance Parameters
Temperature control range: 40 ºC – 99 ºC
Analysis time: ≤ 30 minutes

Applications
Real-time PCR: Absolute quantification
Melting: Labeled probe genotyping
Hi-Res Melting: Mutation scanning
Hi-Res Melting: Unlabeled probe genotyping
Hi-Res Melting: Small amplicon genotyping

Power Requirements
110/220 V, 50/60 Hz, 650 W (auto switching power supply)
Download Download LS32 Technical Notes, References, Manuals, and other Support Items
Request More Info Request More Information
Support LS32 System Support

New Products

The Warfarin Genotyping Reagents and High Sensitivity Master Mix are new products offered by Idaho Technology Inc. Both are compatible with the LightScanner®, LS32™, and
HR-1® instruments.


Free Web Seminars
Somatic Mutation Detection in Primary Tumor Samples

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Featured Audio Slideshow
The LightScanner System: Achieve high throughput mutation discovery and genotyping